Gene expression and physiological responses associated to stomatal functioning in Rosa x hybrida grown at high relative air humidity

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Carvalho, DRA (Autor) Outra; Vasconcelos, MW (Autor) Outra; Lee, S (Autor) Outra; Koning Boucoiran, CFS (Autor) Outra; Vreugdenhil, D (Autor) Outra; Krens, FA (Autor) Outra; Heuvelink, E (Autor) Outra; Carvalho, SMP (Autor) FCUP

Revista

Título: Plant Science

Vol. 253

Páginas: 154-163

ISSN: 0168-9452

Editora: Elsevier

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-00M-4FJ

DOI: 10.1016/j.plantsci.2016.09.018

Abstract (EN): High relative air humidity (RH >= 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 +/- 3%) or high (89 +/- 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (g(s)) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75132, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10