Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coil

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Marani, MM (Autor) Outra; Perez, LO (Autor) Outra; de Araujo, AR (Autor) Outra; Placido, A (Autor) Outra; Sousa, CF (Autor) Outra; Quelemes, PV (Autor) Outra; Oliveira, M (Autor) Outra; Gomes Alves, AG (Autor) Outra; Pueta, M (Autor) Outra; Paula Gameiro (Autor) FCUP; Tomas, AM (Autor) Outra; Delerue Matos, C (Autor) Outra; Eaton, P (Autor) Outra; Camperi, SA (Autor) Outra; Basso, NG (Autor) Outra; Leite, JRDD (Autor) Outra

Revista

Título: Gene

Vol. 605

Páginas: 70-80

ISSN: 0378-1119

Editora: Elsevier

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 23 Citações

Outras Informações

ID Authenticus: P-00M-E3Z

DOI: 10.1016/j.gene.2016.12.020

Abstract (EN): Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 mu M). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11