Involvement of endothelium in the vasorelaxant effects of 3,4-DHPEA-EA and 3,4-DHPEA-EDA, two major functional bioactives in olive oil

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Segade, M (Autor) Outra; Bermejo, R (Autor) Outra; Silva, A (Autor) FCUP; Fatima Paiva Martins (Autor) FCUP; Gil Longo, J (Autor) Outra; Campos Toimil, M (Autor) Outra

Revista

Título: Journal of Functional Foods

Vol. 23

Páginas: 637-646

ISSN: 1756-4646

Editora: Elsevier

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00K-AVY

DOI: 10.1016/j.jff.2016.03.024

Abstract (EN): The olive oil polyphenols 3,4-DHPEA-EA and 3,4-DHPEA-EDA displayed an endothelium dependent vasorelaxant effect in rat aorta, starting at similar to 1 mu M and abolished by N-G-nitro-L-arginine (L-NA) or N-acetylcysteine, and an endothelium-independent vasorelaxant effect, starting at similar to 10 mu M. Hydroxytyrosol only presented an endothelium-independent effect at 100 mu M. DHPEA-EA and 3,4-DHPEA-EDA, but not hydroxytyrosol, also increased NO generation within endothelial cells. At higher concentrations, the three compounds reduced argininevasopressin-induced increase of cytosolic Ca2+ concentration ([Ca2+](c)) in vascular myocytes. By UV-visible spectroscopy, we found that these polyphenols undergo autoxidative processes in organ-bath conditions. Thus, 3,4-DHPEA-EA and 3,4-DHPEA-EDA have an endothelium-dependent vasorelaxant effect caused by an enhanced NO production, probably through a redox mechanism within endothelial cells and an endothelium-independent vasorelaxant effect mediated by a reduction of agonist-induced [Ca2+](c) increase in vascular myocytes. Bearing in mind the plasmatic concentrations of these polyphenols following dietary intake of olive oil, these effects could modulate vascular tone in vivo.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10