Tethering antimicrobial peptides onto chitosan: Optimization of azide-alkyne "click" reaction conditions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Barbosa, M (Autor) Outra; Nuno Vale (Autor) ICBAS; Costa, FMTA (Autor) Outra; Martins, MCL (Autor) Outra; Paula Gomes (Autor) FCUP

Revista

Título: Carbohydrate Polymers

Vol. 165

Páginas: 384-393

ISSN: 0144-8617

Editora: Elsevier

Indexação

ISI Web of Knowledge - 62 Citações

Scopus - 61 Citações

Outras Informações

ID Authenticus: P-00M-QKQ

DOI: 10.1016/j.carbpol.2017.02.050

Abstract (EN): Antimicrobial peptides (AMP) are promising alternatives to classical antibiotics, due to their high specificity and potency at low concentrations, and low propensity to elicit pathogen resistance. Immobilization of AMP onto biomaterials is an emergent field of research, towards creation of novel antimicrobial materials able to avoid formation of biofilms on the surfaces of medical devices. Herein, we report the chemical route towards one such material, where chitosan was used as biocompatible carrier for the covalent grafting of Dhvar-5, a well-known potent AMP, via the chemoselective ("click") Cu(I)-catalyzed azidealkyne cycloaddition (CuAAC). The material's structure, as well as peptide loading, were confirmed by Fourier-transformed infra-red (FT-IR) and X-ray photoelectron (XPS) spectroscopies, and by Amino Acid Analysis (AAA), respectively. Results herein reported demonstrate that, with proper optimization, the "click" CuAAC is an attractive approach for the tethering of AMP onto chitosan, in order to create novel antimicrobial materials potentially valuable for biomedical applications.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10