Changes in growth, chlorophyll content and peroxidase and alpha-amylase activities in sunflower calli after UV-C irradiation

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

Conde, P (Autor) Outra; Sousa, A (Autor) Outra; Santos, S (Autor) Outra; Dos Santos, CV (Autor) Outra; Caldeira, G (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: BELGIAN JOURNAL OF BOTANY

Vol. 132

Páginas: 35-42

ISSN: 0037-9557

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-001-5CX

Abstract (EN): Sunflower (Helianthus annuus L. cv. Girapac SH222) calli were induced and grown on modified MURASHIGE & SKOOG (1962) medium. Two-month-old calli were exposed for 15 minutes to four UV-C radiation treatments : one irradiance of 3.170 mW.cm(-2) for one day (treatment T1); one irradiance of 3.170 mW.cm-2 every day for seven days (treatment T2); one irradiance of 5.350 mW.cm(-2) for one day (treatment T3) and one irradiance of 5.350 mW.cm(-2) for seven days (treatment T4). Control calli were maintained under white light conditions (treatment T0). Eight-day-old sunflower plants, grown under greenhouse conditions, were also exposed to UV-C radiation (T1 and T2). Irradiated calli soon became dark and with necrotic areas, but they were more tolerant to UV irradiation than plants, since they could sustain growth. Ca2+ and K+ contents were not significantly affected in irradiated calli cells. On a calli fresh weight basis, T1 and T2 treatments resulted in a significant increase of soluble protein levels. Calli cells from UV treatments suffered a significant reduction in guaiacol peroxidase activity and an increase in alpha-amylase activity. In control calli alpha-amylase activity decreased with time. The present study shows that sunflower calli are more tolerant to UV rays than plants, and demonstrates interactions between UV-C radiation and peroxidase and alpha-amylase activities.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8