Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Pinto, G (Autor) Outra; Silva, S (Autor) Outra; Loureiro, J (Autor) Outra; Costa, A (Autor) Outra; Dias, MC (Autor) Outra; Araujo, C (Autor) Outra; Neves, L (Autor) Outra; Santos, C (Autor) Outra

Revista

Título: Trees - Structure and Function

Vol. 25

Páginas: 383-392

ISSN: 0931-1890

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-002-R32

DOI: 10.1007/s00468-010-0513-y

Abstract (EN): This paper reports the complete process from secondary emblings (SE-derived plants) regeneration to acclimatization of Eucalyptus globulus and describes histocytological changes that occur in leaves from in vitro to ex vitro acclimatization for a 3-month period. After elongation, plants were transferred to pots with sterilized peat:perlite and acclimatized in a phytotron, with progressive reduction of RH and increase of light intensity. Histocytological analyses were performed in fixed material using light microscopy and ultrastructural changes followed by electron microscopy (SEM and TEM). The protocol used allowed the successful acclimatization of the emblings. Plants looked morphologically normal and FCM screening revealed no ploidy or DNA content abnormalities. Histocytological analyses showed significant changes along time, mostly in stomata shape and aperture, starch reserves, chloroplast morphology and mesophyll differentiation. This is the first report concerning emblings acclimatization to ex vitro conditions in Eucalyptus. It was clearly demonstrated that during acclimatization emblings suffered profound changes in leaf morphology in order to successfully adapt to ex vitro conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10