Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Correia, B (Autor) Outra; Luis Rodriguez, JL (Autor) Outra; Valledor, L (Autor) Outra; Almeida, T (Autor) Outra; Santos, C (Autor) Outra; Jesus Canal, MJ (Autor) Outra; Pinto, G (Autor) Outra

Revista

Título: Journal of Plant Physiology

Vol. 171

Páginas: 399-406

ISSN: 0176-1617

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-009-8Q0

DOI: 10.1016/j.jplph.2013.12.004

Abstract (EN): Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks' distribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant performance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 degrees C showed abundance alterations in the tested genes; at 45 degrees C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 degrees C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8