Dysregulated expression of microRNA-150 in human papillomavirus-induced lesions of K14-HPV16 transgenic mice

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Joana M. O. Santos (Autor) Outra; Mara Fernandes (Autor) Outra; Rita Araújo (Autor) Outra; Hugo Sousa (Autor) Outra; Joana Ribeiro (Autor) Outra; Margarida M. S. M. Bastos (Autor) FEUP; Paula A. Oliveira (Autor) Outra; Diogo Carmo (Autor) Outra; Fátima Casaca (Autor) Outra; Sandra Silva (Autor) Outra; Ana L. Teixeira (Autor) Outra; Rui M. Gil da Costa (Autor) FEUP; Rui Medeiros (Autor) Outra

Revista

Título: Life Sciences

Vol. 175

Páginas: 31-36

ISSN: 0024-3205

Editora: Elsevier

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 12 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00M-MXV

DOI: 10.1016/j.lfs.2017.03.008

Abstract (EN): Aims: High-risk human papillomavirus (HPV) infection is one of the major causes of infection-related cancers worldwide. MicroRNAs (miRNAs) are a family of non-coding RNAs (ncRNAs), whose dysregulated levels may cause an aberrant expression of genes involved in oncogenic pathways and consequently lead to cancer development. This is the case of the miRNA-150 (miR-150), whose expression in HPV-induced lesions remains unclear and the present work aims to clarify it. We employed K14-HPV16 mice, which express the early genes of HPV16 in basal keratinocytes, leading to the development of hyperplastic and dysplastic skin lesions and squamous cell carcinomas, and are a representative model of HPV-induced cancers. Main methods: In order to evaluate the expression of miR-150 in HPV-induced lesions, we performed qPCR in wild-type mice (HPV-/-) and in skin lesions of K14-HPV16 transgenic mice (HPV+/-). Matched skin samples were analyzed histologically. Key findings: 24-26 weeks-old HPV+/- mice showed diffuse epidermal hyperplasia and focal dysplasia in a hyperplastic background (31.8% incidence), but 28-30 weeks-old HPV+/- mice presented higher incidence of dysplasia (100.0%). MiR-150 was upregulated in HPV-/- mice when compared with HPV-/- mice (p < 0.001). MiR150 was also overexpressed in diffuse dysplastic lesions when compared with hyperplastic lesions (p = 0.005). Significance: The present results suggest that miR-150 is overexpressed in HPV-induced lesions in this model and its expression seems to increase with lesion progression, along the process of multi-step carcinogenesis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6