Paired related homeobox protein-like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Monteiro, CB (Autor) Outra; Costa, MF (Autor) Outra; reguenga, c (Autor) FMUP; Lima, D (Autor) Outra; Castro, DS (Autor) Outra; Filipe Monteiro (Autor) FMUP

Revista

Título: FEBS Letters

Vol. 588

Páginas: 3475-3482

ISSN: 0014-5793

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Outras Informações

ID Authenticus: P-009-T2H

DOI: 10.1016/j.febslet.2014.08.006

Abstract (EN): The homeodomain factor paired related homeobox protein-like 1 (Prrxl1) is crucial for proper assembly of dorsal root ganglia (DRG)-dorsal spinal cord (SC) pain-sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue-specific binding of Prrxl1. Transcriptional assays confirm the identified regions can mediate repression by Prrxl1, while gain-of-function studies in Prrxl1 expressing ND7/23 cells indicate Prrxl1 can down-regulate its own expression. Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8