Diversity and antibiotic resistance of Acinetobacter spp. in water from the source to the tap

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Carlos Narciso da Rocha (Autor) Outra; Ivone Vaz Moreira (Autor) FEUP; Liselott Svensson Stadler (Autor) Outra; Edward R. B. Moore (Autor) Outra; Célia M. Manaia (Autor) Outra

Revista

Título: Applied Microbiology and Biotechnology

Vol. 97 Nº 1

Páginas: 329-340

ISSN: 0175-7598

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 21 Citações

Projetos Associados

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Outras Informações

ID Authenticus: P-002-1WN

DOI: 10.1007/s00253-012-4190-1

Abstract (EN): Acinetobacter spp. are ubiquitous bacteria in the environment. Acinetobacter spp. isolated from a municipal drinking water treatment plant and from connected tap water were identified to the species level on the basis of rpoB gene partial sequence analysis. Intraspecies variation was assessed based on the analysis of partial sequences of housekeeping genes (rpoB, gyrB, and recA). Antibiotic resistance was characterized using the disk diffusion method and isolates were classified as wild or non-wild type (non-WT), according to the observed phenotype. The strains of Acinetobacter spp. were related to 11 different validly published species, although three groups of isolates, presenting low rpoB sequence similarities with previously described species, may represent new species. Most of the isolates were related to the species A. johnsonii and A. lwoffii. These two groups, as well as others related to the species A. parvus and A. tjernbergiae, were detected in the water treatment plant and in tap water. Other strains, related to the species A. pittii and A. beijerinckii, were isolated only from tap water. Most of the isolates (80 %) demonstrated wild type (WT) to all of the 12 antibiotics tested. Non-WT for tetracycline, meropenem, and ceftazidime, among others, were observed in water treatment plant or in tap water samples. Although, in general, this study suggests a low prevalence of acquired antibiotic resistance in water Acinetobacter spp., the potential of some species to acquire and disseminate resistance via drinking water is suggested.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12