Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC-UV

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Rodrigues, AS (Autor) Outra; Lopes, AR (Autor) Outra; Leao, A (Autor) Outra; Couceiro, A (Autor) Outra; Sarmento Ribeiro, ABS (Autor) Outra; Ramos, F (Autor) Outra; Noronha da Silveira, MIN (Autor) Outra; de Oliveira, CR (Autor) Outra

Revista

Título: Journal of Chromatographic Science

Vol. 47 Nº 2

Páginas: 387-391

ISSN: 0021-9665

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-003-KEJ

Abstract (EN): A high-performance liquid chromatography-UV methodology (λ= 230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50mM, pH 3.2 ± 0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 οg/mL and 5-100 οg/mL intervals, and detection (0.19 ×0.51 οg/mL) and quantification (0.63 ×1.7 οg/mL) limits for vincristine and doxorubicin, respectively.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5