Recombination detection in Aspergillus fumigatus through single nucleotide polymorphisms typing

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Teixeira, J (Autor) Outra; Amorim, A (Autor) FCUP; Araujo, R (Autor) Outra

Revista

Título: Environmental Microbiology Reports

Vol. 7

Páginas: 881-886

ISSN: 1758-2229

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00K-0CC

DOI: 10.1111/1758-2229.12321

Abstract (EN): The first evidence of sexual reproduction in Aspergillus fumigatus was reported in 2009. Nevertheless, it remains difficult to understand how A. fumigatus is able to reproduce through this mode in its natural environment and how frequently this occurs. The aim of this study was to analyse single nucleotide polymorphisms (SNPs) in a set of environmental and clinical isolates of A. fumigatus to detect signatures of recombination. A group of closely related Portuguese A. fumigatus isolates was characterized by mating type and the genetic diversity of the intergenic regions, microsatellites and multilocus sequence typing (MLST) genes. A group of 19 SNPs, organized in nine association groups and inherited in blocks, was identified and compared. Several variations on the association panel were detected on reference isolates of A. fumigatus AF293 and A1163, the sequence types available at MLST database and six clinical and environmental Portuguese isolates. About one to four haplotype disruptions were observed per isolate. Considering clinical and environmental isolates, sexual reproduction seems to occur more frequently than previously admitted in A. fumigatus. In this study, a practical SNP approach is proposed for detection of recombination events in larger collections of A. fumigatus.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6