Estradiol modulates Na+-dependent HCO3- transporters altering intracellular pH and ion transport in human Sertoli cells: A role on male fertility?

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Bernardino, RL (Autor) Outra; Costa, AR (Autor) Outra; Martins, AD (Autor) Outra; Silva, J (Autor) Outra; barros, a (Autor) FMUP; sousa, m (Autor) ICBAS; Sa, R (Autor) Outra; Alves, MG (Autor) Outra; Oliveira, PF (Autor) ICBAS

Revista

Título: Biology of the Cell

Vol. 108

Páginas: 179-188

ISSN: 0248-4900

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 25 Citações

Scopus - 25 Citações

Outras Informações

ID Authenticus: P-00K-NNA

DOI: 10.1111/boc.201500094

Abstract (EN): Background informationInfertile men often present deregulation of serum estrogen levels. Notably, high levels of estradiol (E2) are associated with low sperm production and quality. Sertoli cells (SCs) are responsible for spermatogenesis maintenance and are major targets for the hormonal signalling that regulates this complex process. ResultsIn this study, we used primary cultures of human SCs and studied the localisation, expression and functionality of the Na+-dependent HCO3- transporters by confocal microscopy, immunoblot, epifluorescence and voltage clamp after 24 h of exposure to E2 (100 nM). All studied transporters were identified in human SCs. In E2-treated human SCs, there was an increase in NBCn1, NBCe1 and NDCBE protein levels, as well as an increase in intracellular pH and a decrease in transcellular transport. ConclusionsWe report an association between increased levels of E2 and the expression/function of Na+-dependent HCO3- transporters in human SCs. Our results provide new evidence on the mechanisms by which E2 can regulate SCs physiology and consequently spermatogenesis. These mechanisms may have an influence on male reproductive potential and help to explain male infertility conditions associated with estrogen deregulation. SignificanceExposure to E2 increased human SCs intracellular pH. E2 is a modulator of ionic transcellular transport in human SCs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11