Antifungal activity of local anesthetics against Candida species

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

pina-vaz, c (Autor) FMUP; rodrigues, ag (Autor) FMUP; Sansonetty, F (Autor) Outra; Martinez De Oliveira, J (Autor) Outra; Fonseca, AF (Autor) Outra; Mardh, PA (Autor) Outra

Revista

Título: Infectious Diseases in Obstetrics and Gynecology

Vol. 8

Páginas: 124-137

ISSN: 1064-7449

Editora: Hindawi

Indexação

Scopus - 53 Citações

Outras Informações

ID Authenticus: P-007-QAH

Abstract (EN): Objective: To evaluate the activity of benzydamine, lidocaine, and bupivacaine, three drugs with local anesthetic activity, against Candida albicans and non-albicans strains and to clarify their mechanism of activity. Methods: The minimal inhibitory concentration (MIC) was determined for 20 Candida strains (18 clinical isolates and two American Type Culture Collection strains). The fungistatic activity was studied with the fluorescent probe FUN-1 and observation under epifluorescence microscopy and flow cytometry. The fungicidal activity of the three drugs was assayed by viability counts. Membrane alterations induced in the yeast cells were evaluated by staining with propidium iodide, by quantitation of intracellular K+ leakage and by transmission electron microscopy of intact yeast cells and prepared spheroplasts. Results: The MIC ranged from 12.5-50.0 ¿g/mL, 5.0-40.0 mg/mL, and 2.5-10.0 mg/mL for benzydamine, lidocaine, and bupivacaine, respectively. The inhibitory activity, of these concentrations could be detected with the fluorescent probe FUN-1 after incubation for 60 minutes. A very fast fungicidal activity was shown by 0.2, 50, and 30 mg/mL of benzydamine, lidocaine, and bupivacaine, respectively. Conclusions: At lower concentrations, the tested drugs have a fungistatic activity, due to yeast metabolic impairment, while at higher concentrations they are fungicidal, due to direct damage to the cytoplasmic membrane. (C) 2000 Wiley-Liss, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica