Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Almeida, CR (Autor) Outra; Vasconcelos, DP (Autor) Outra; Goncalves, RM (Autor) ICBAS; Mário Adolfo Barbosa (Autor) FEUP

Revista

Título: Journal of the Royal Society Interface

Vol. 9

Páginas: 261-271

ISSN: 1742-5689

Editora: The Royal Society

Indexação

ISI Web of Knowledge - 52 Citações

Scopus - 55 Citações

Outras Informações

ID Authenticus: P-002-D3E

DOI: 10.1098/rsif.2011.0357

Abstract (EN): An exacerbated inflammatory response questions biomaterial biocompatibility, but on the other hand, inflammation has a central role in the regulation of tissue regeneration. Therefore, it may be argued that an 'ideal' inflammatory response is crucial to achieve efficient tissue repair/regeneration. Natural killer (NK) cells, being one of the first populations arriving at an injury site, can have an important role in regulating bone repair/regeneration, particularly through interactions with mesenchymal stem/stromal cells (MSCs). Here, we studied how biomaterials designed to incorporate inflammatory signals affected NK cell behaviour and NK cell-MSC interactions. Adsorption of the pro-inflammatory molecule fibrinogen (Fg) to chitosan films led to a 1.5-fold increase in adhesion of peripheral blood human NK cells, without an increase in cytokine secretion. Most importantly, it was found that NK cells are capable of stimulating a threefold increase in human bone marrow MSC invasion, a key event taking place in tissue repair, but did not affect the expression of the differentiation marker alkaline phosphatase (ALP). Of significant importance, this NK cell-mediated MSC recruitment was modulated by Fg adsorption. Designing novel biomaterials leading to rational modulation of the inflammatory response is proposed as an alternative to current bone regeneration strategies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11