Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Judite N. Barbosa (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP; Artur Águas (Autor) ICBAS

Revista

Título: Biomaterials

Vol. 25 Nº 13

Páginas: 2557-2563

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 51 Citações

Scopus - 54 Citações

Outras Informações

ID Authenticus: P-000-A18

DOI: 10.1016/j.biomaterials.2003.09.047

Abstract (EN): The acute inflammatory response and the adhesion of cells to self-assembled monolayers (SAMs) of well-defined surface chemistry was studied in vivo using a rodent air-pouch model of inflammation. SAMs with three different terminal functional groups (OH, COOH and CH3) were implanted in subcutaneous air pouches induced in BALB/c mice. After 24 h, inflammatory cells were recovered from the air pouches and the implants were removed and prepared for observation by scanning electron microscopy (SEM). The implants coated with OH and CH3, were found to cause the highest recruitment of inflammatory cells into the subcutaneous pouches. Polymorphonuclear neutrophils (PMNs) leukocytes predominated over mononuclear cells in inflammatory exudates of SAMs-coated implants, the opposite being found in uncoated implants (controls). CH3-coated implants induced the highest number of inflammatory cells and also the largest percentage of PMNs seen in the subcutaneous pouches. Control and OH-covered implants presented the higher densities of attached inflammatory cells detected by SEM. In contrast, the CH3-coated implants showed a very low density of cells adherent to the implant surface. We conclude that the chemical nature and the degree of hydrophobicity of the surface of implants modulate both the local acute inflammatory reaction and the adhesion of leukocytes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7