Influence of nitrogen/carbon ratio and complementary sugars on dextransucrase production by Leuconostoc mesenteroides NRRL B512(f).

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

Lopretti, M (Autor) Outra; Martinez, E (Autor) Outra; Torres, L (Autor) Outra; Perdomo, R (Autor) Outra; Santos, M (Autor) Outra; Alírio Rodrigues (Autor) FEUP

Revista

Título: Process Biochemistry

Vol. 34 Nº 9

Páginas: 879-884

ISSN: 1359-5113

Editora: Elsevier

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-001-38Z

DOI: 10.1016/s0032-9592(99)00020-5

Abstract (EN): The influence of the nitrogen/carbon ratio was studied during fermentation with Leuconostoc mesenteroides NRRL B512(f) using sucrose as substrate. The enzyme concentration was measured through activity tests and radiotracer tests with [C-14]phenylalanine. In batch fermentation a slowing down in the rate of enzyme synthesis was observed with a decrease in the nitrogen/carbon ratio. Addition of pulses of nitrogen when the nitrogen/carbon ratio decreased allowed a constant production rate of enzyme and lower fermentation time. The influence of complementary sugars was also addressed. Lactose inhibited enzyme production. When galactose was used the yield was the same as in the fermentation with sucrose alone, but with a different production rate. Maltose favoured the synthesis of dextransucrase.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6