Characterization of cholinesterase from guppy (Poecilia reticulata) muscle and its in vitro inhibition by environmental contaminants

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Garcia, LM (Autor) Outra; Castro, B (Autor) Outra; Ribeiro, R (Autor) Outra; Guilhermino, L. (Autor) ICBAS

Revista

Título: Biomarkers

Vol. 5

Páginas: 274-284

ISSN: 1354-750X

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 43 Citações

Outras Informações

ID Authenticus: P-000-ZJ5

DOI: 10.1080/135475000413827

Abstract (EN): With a view to using the cholinesterase (ChE) activity from guppy (Poecilia reticulata) muscle as a biomarker, the objectives of this work were: (i) to characterize the soluble cholinesterases present in muscle homogenate using different substrates and specific inhibitors, (ii) to determine the normal range of activity in non-exposed individuals and (iii) to investigate the in vitro effects of two common environmental contaminants, copper sulphate and dodecylbenzene sulphonic acid sodium salt (DBS) on ChE activity. The rate of substrate hydrolysis of P. reticulata ChE decreased in the order acetylthiocholine, propionylthiocholine and butyrylthiocholine. Inhibition by excess of substrate was observed at concentrations higher than 1.28 mM. Furthermore, eserine sulphate and 1,5-bis(4-allyldimethylammoniumphenyl) -pentan-3-one (BW284C51) significantly inhibited the enzyme activity at low concentrations (mM range) and N,N'-diisopropylphosphorodiamic acid (iso-OMPA) had no significant effect up to 8 mM. These findings suggest that the enzyme measured in this study is acetylcholinesterase. The activity determined in non-exposed fish was 145.1+/-44.7 SD U mg(-1) protein. The common environmental contaminants copper and DBS significantly inhibited P. reticulata ChE at concentrations that can be ecologically relevant.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11