Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Parreira, P (Autor) Outra; Shi, Q (Autor) Outra; Magalhaes, A (Autor) Outra; Celso Reis (Autor) ICBAS; Bugaytsova, J (Autor) Outra; Boren, T (Autor) Outra; Leckband, D (Autor) Outra; Martins, MCL (Autor) Outra

Revista

Título: Journal of the Royal Society Interface

Vol. 11

Páginas: 20141040-20141040

ISSN: 1742-5689

Editora: The Royal Society

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-009-XFP

DOI: 10.1098/rsif.2014.1040

Abstract (EN): The strength of binding between the Helicobacter pylori blood group antigen-binding adhesin (BabA) and its cognate glycan receptor, the Lewis b blood group antigen (Leb), was measured by means of atomic force microscopy. High-resolution measurements of rupture forces between single receptor-ligand pairs were performed between the purified BabA and immobilized Leb structures on self-assembled monolayers. Dynamic force spectroscopy revealed two similar but statistically different bond populations. These findings suggest that the BabA may form different adhesive attachments to the gastric mucosa in ways that enhance the efficiency and stability of bacterial adhesion.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7