Increased spontaneous release of tumour necrosis factor-alpha by alveolar macrophages from wheezy infants

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

Inês Azevedo (Autor) FMUP; deBlic, J (Autor) Outra; Dumarey, CH (Autor) Outra; Scheinmann, P (Autor) Outra; Vargaftig, BB (Autor) Outra; Bachelet, M (Autor) Outra

Revista

Título: European Respiratory Journal

Vol. 10

Páginas: 1767-1773

ISSN: 0903-1936

Editora: European Respiratory Society

Indexação

ISI Web of Knowledge - 42 Citações

Scopus - 55 Citações

Outras Informações

ID Authenticus: P-008-Q6P

DOI: 10.1183/09031936.97.10081767

Abstract (EN): We determined if alveolar macrophages (AMs) from infants with severe recurrent wheezing episodes release increased amounts of tumour necrosis factor-alpha (TNF-alpha), as described in adults with asthma, We compared TNF-alpha release by unstimulated and lipopolysaccharide-stimulated AMs obtained by bronchoalveolar lavage in 13 wheezy and seven nonwheezy infants (aged 6-36 months) and analysed its regulation by dexamethasone. Metabolites in cell supernatants were quantified by enzyme-linked immunosorbent assay (ELISA) (TNF-alpha) or radioimmunoassay (thromboxane B-2 and prostaglandin E-2). Comparison of results was performed by the Mann-Whitney U-test and values were expressed as median (interquartile range) in ng.10(6) cells(-1). Resting AMs from wheezy infants released larger amounts of TNF-alpha and thromboxane B-2 as compared to controls: 2.67 (0.89-8.33) vs 0.48 (0.25-1.08) and 75.63 (38.07-158.91) vs 10.03 (7.36-76.08), respectively (p<0.05). When stimulated overnight with bacterial lipopolysaccharide, AMs from both groups released similar amounts of metabolites, Dexamethasone induced a consistent inhibition of the lipopolysaccharide-stimulated release of all the mediators, Our results show that alveolar macrophages from wheezy infants are activated to release increased amounts of tumour necrosis factor-alpha, as in asthma, and suggest that infants with recurrent wheezing may eventually benefit from treatment with glucocorticoids.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7