Mucins MUC16 and MUC1 are major carriers of SLe(a) and SLe(x) in borderline and malignant serous ovarian tumors

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Ricardo, S (Autor) Outra; Marcos Silva, L (Autor) Outra; Valente, C (Autor) Outra; Coelho, R (Autor) Outra; Gomes, R (Autor) Outra; David L (Autor) FMUP

Revista

Título: Virchows Archiv

Vol. 468

Páginas: 715-722

ISSN: 0945-6317

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 14 Citações

Outras Informações

ID Authenticus: P-00K-B8K

DOI: 10.1007/s00428-016-1929-6

Abstract (EN): Mucins are heavily glycosylated proteins overexpressed and associated with truncated or sialylated glycans upon malignant transformation. We previously identified a panel of four glyco-mucin profiles (MUC16/Tn, MUC16/STn, MUC1/Tn, and MUC1/STn) with 100 % specificity and 100 % positive predictive value for detection of borderline/malignant serous tumors of the ovary, using proximity ligation assay (PLA). In the present work, using the same method, we studied other mucin glycosylation profiles that might add relevant information for diagnostic purposes. We used PLA probes to MUC16, MUC1, sialyl Lewis(a) (SLe(a)), and sialyl Lewis(x) (SLe(x)) to study a series of 39 ovarian serous tumors (14 adenocarcinomas, 10 borderline ovarian tumors (BOTs), and 15 cystadenomas). Our results demonstrated that, in adenocarcinomas and BOTs, the major carriers of SLe(a) and SLe(x) are MUC16 and/or MUC1 (100 and 92% for SLe(a) and 64 and 70% for SLe(x), respectively). In cystadenomas, SLe(a) and SLe(x) are mainly carried by unidentified proteins (85 and 78 %, respectively). Our study identified, for the first time, the major protein carriers of SLe(a) and SLe(x) in ovarian adenocarcinomas and BOTs, MUC1 and MUC16, and also that distinct unidentified carriers are involved in cystadenomas. These results emphasize the relevance of multiple biomarker recognition provided by multiplex assays, such as PLA, to enhance sensitivity and specificity of serum and tissue assays.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8