Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I expression in gastric carcinoma cell lines

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Marcos, NT (Autor) Outra; Cruz, A (Autor) Outra; Santos-Silva F (Autor) Outra; Raquel Almeida (Autor) Outra; David L (Autor) FMUP; Mandel, U (Autor) Outra; Clausen, H (Autor) Outra; von Mensdorff Pouilly, S (Autor) Outra; Celso Reis (Autor) Outra

Revista

Título: Journal of Histochemistry and Cytochemistry

Vol. 51

Páginas: 761-771

ISSN: 0022-1554

Editora: Histochemical Society

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 43 Citações

Outras Informações

ID Authenticus: P-000-GET

DOI: 10.1177/002215540305100607

Abstract (EN): Mucin O-glycosylation in cancer is characterized by aberrant expression of immature carbohydrate structures leading to exposure of simple mucin-type carbohydrate antigens and peptide epitopes. Glycosyltransferases controlling the initial steps of mucin O-glycosylation are responsible for the altered glycosylation observed in cancer. We studied the expression in gastric cell lines of six UDP-GaINAc:polypeptide N-acetylgalactosaminyl-transferases (GaINAc-T1, T2, T3, T4, T6, T11) that catalyze the initial key step in the regulation of mucin O-glycosylation, the transfer of GaINAc from UDP-GaINAc to serine and threonine residues. We also studied the expression of ST6GaINAc-I, the enzyme responsible for the synthesis of Sialyl-Tn antigen (NeuAcalpha2,6GaINAc) and the ST3GaI-I, the enzyme responsible for the synthesis of Sialyl-T antigen (NeuAcalpha2,3GaIbeta1,3GaINAc). This study was done using specific monoclonal antibodies, enzymatic assays, and RT-PCR. Our results showed that GaINAc-T1, -T2, and -T3 have an ubiquitous expression in all gastric cell lines, whereas GaINAc-T4, -T6, and -T11 show a restricted expression pattern. The immunoreactivity with MAb VU-2-G7 suggests that, apart from GaINAc-T4, another GaINAc transferase is involved in the glycosylation of the Thr in the PDTR region of the MUC1 tandem repeat. The expression of ST3GaI-I correlates with the expression of the Sialyl-T antigen in gastric cell lines and in the control cell lines studied. The expression of ST6GaINAc-I is low in gastric cell lines, in accordance with the low/absent expression of the Sialyl-Tn antigen.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11