Expanding allelic diversity of Helicobacter pylori vacA

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

van Doorn, LJ (Autor) Outra; Figueiredo C (Autor) Outra; Sanna, R (Autor) Outra; Pena, S (Autor) Outra; Midolo, P (Autor) Outra; Ng, EKW (Autor) Outra; Atherton, JC (Autor) Outra; Blaser, MJ (Autor) Outra; Quint, WGV (Autor) Outra

Revista

Título: Journal of Clinical Microbiology

Vol. 36

Páginas: 2597-2603

ISSN: 0095-1137

Editora: American Society for Microbiology

Indexação

ISI Web of Knowledge - 228 Citações

Scopus - 257 Citações

Outras Informações

ID Authenticus: P-001-76Z

Abstract (EN): The diversity of the gene encoding the vacuolating cytotoxin (vacA) of Helicobacter pylori was analyzed in 98 isolates obtained from different geographic locations. The studies focused on variation in the previously defined s and m regions of vacA, as determined bg PCR and direct sequencing. Phylogenetic analysis revealed the existence of four distinct types of s-region alleles: aside from the previously described sla, s1b, and s2 allelic types, a novel subtype, designated sie, was found. Subtype sie was observed exclusively in isolates from East Asia and appears to be the major sl allele in that part of the world. Three different allelic forms (m1, m2a, and m2b) were detected in the m region. On the basis of sequence alignments, universal PCR primers that allow effective amplification of the s and m regions from H. pylori isolates from all over the world were defined. Amplimers were subsequently analyzed by reverse hybridization onto a line probe assay (LiPA) that allows the simultaneous and highly specific hybridization of the different vacA s- and m-region alleles and tests for the presence of the cytotoxin-associated gene (cagA). This PCR-LiPA method permits rapid analysis of the vacA and cagA status of H. pylori strains for clinical and epidemiological studies and will facilitate identification of any further variations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7