Production of microparticles of molinate degrading biocatalysts using the spray drying technique

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Ana R. Lopes (Autor) FEUP; Vera M. Sousa (Autor) FEUP; Berta N. Estevinho (Autor) FEUP; José P. Leite (Autor) Outra; Nuuno F. F. Moreira (Autor) Outra; Luís Gales (Autor) Outra; Fernando Rocha (Autor) FEUP; Olga C. Nunes (Autor) FEUP

Revista

Título: Chemosphere

Vol. 161

Páginas: 61-68

ISSN: 0045-6535

Editora: Elsevier

Indexação

ISI Web of Knowledge - 9 Citações

ISI Web of Science

Scopus - 9 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias

Projetos Associados

PTDC/AAG-TEC/3909/2012|FCOMP-01-0124-FEDER-027883 - Caracterização e aplicação de uma nova enzima (hidrolase do molinato, MolA) em processos de biorremediação

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00K-N2C

DOI: 10.1016/j.chemosphere.2016.07.006

Abstract (EN): Previous studies demonstrated the capability of mixed culture DC1 to mineralize the thiocarbamate herbicide molinate through the activity of molinate hydrolase (MolA). Because liquid suspensions are not compatible with long-term storage and are not easy to handle when bioremediation strategies are envisaged, in this study spray drying was evaluated as a cost-effective method to store and transport these molinate biocatalysts. Microparticles of mixed culture DC1 (DC1) and of cell free crude extracts containing MolA (MA) were obtained without any carrier polymer, and with calcium alginate (CA) or modified chitosan (MCt) as immobilizing agents. All the DC1 microparticles showed high molinate degrading activity upon storage for 6 months, or after 9 additions of ¿0.4 mM molinate over 1 month. The DC1-MCt microparticles were those with the highest survival rate and lowest heterogeneity. For MA microparticles, only MA-MCt degraded molinate. However, its Vmax was only 1.4% of that of the fresh cell free extract (non spray dried). The feasibility of using the DC1-MCt and MA-MCt microparticles in bioaugmentation processes was assessed in river water microcosms, using mass (g):volume (L) ratios of 1:13 and 1:0.25, respectively. Both type of microparticles removed ¿65¿75% of the initial 1.5 mg L¿1 molinate, after 7 days of incubation. However, only DC1-MCt microparticles were able to degrade this environmental concentration of molinate without disturbing the native bacterial community. These results suggest that spray drying can be successfully used to produce DC1-MCt microparticles to remediate molinate polluted sites through a bioaugmentation strategy. © 2016 Elsevier Ltd

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8