Ultrastructural and cytochemical aspects of the basophilic cells in the hepatopancreas of Aplysia depilans (Mollusca, Opisthobranchia)

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

lobo-da-cunha, a (Autor) ICBAS

Revista

Título: TISSUE & CELL

Vol. 31

Páginas: 8-16

ISSN: 0040-8166

Editora: CHURCHILL LIVINGSTONE

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-001-583

DOI: 10.1054/tice.1998.0014

Abstract (EN): The basophilic cells of Aplysia depilans have a pyramidal shape and a large nucleus usually located near the center or in the basal half of the cell. The nucleus possesses several clumps of condensed chromatin and a prominent nucleolus. The great profusion of rough endoplasmic reticulum cisterns in a major feature of these cells. Secretion granules are accumulated in the apical zone, and arylsulphatase was detected in some of them. In some basophilic cells a very substantial part of the cell volume was occupied by clear vacuoles, some of them reaching 9 mu m However, in other cells only a few vacuoles were observed. Probably the cells with just a few vacuoles are still young, and after a progressive accumulation, the vacuoles become abundant in old cells. The presence of a dark nucleus in the cells with a large number of vacuoles suggests that they are in a final stage of their life. Arylsulphatase was detected in the vacuoles and also in small secondary lysosomes containing substances in digestion. Bundles of tubules with 50 nm in diameter were found within some cisterns of rough endoplasmic reticulum, A cell fraction enriched in mannitol oxidase, extracted from the hepatopancreas of a terrestrial slug, consisted in very similar tubular structures. Using a histochemical method, mannitol oxidase was detected in the basophilic cells of A. depilans, and it may be associated with the tubular structures of the endoplasmic reticulum, This is the first report of mannitol oxidase in opisthobranch molluscs, Almost spherical peroxisomes with a small nucleoid were abundant in these cells. The nucleoids presented a rectangular section, but a crystalline structure was not evident. The peroxisomes were stained after the cytochemical detection of catalase activity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9