THE GOLGI-APPARATUS OF THE CILIATED PROTOZOAN ICHTHYOPHTHIRIUS-MULTIFILIIS - A CYTOCHEMICAL STUDY

• Tipo: Artigo em Revista Científica Internacional
• Data: 1994

Autores

lobo-da-cunha, a (Autor) ICBAS; AZEVEDO, C (Autor) Outra

Revista

Título: European Journal of Protistology

Vol. 30

Páginas: 97-103

ISSN: 0932-4739

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-001-KCW

Abstract (EN): The trophozoites of the ciliated protozoon Ichthyophthirius multifiliis, a fish parasite, possess many small dictyosomes. Each one is associated with a modified cisterna of the endoplasmic reticulum. AcPase, TPPase and NDPase activities were located at the trans face of these dictyosomes. In the cell cortex, thick-membrane cisternae with coated dilated rims are abundant and possess AcPase, TPPase and NDPase activities. Our results suggest that the thick membrane cisternae of ciliated protozoa may be a terminal compartment of the Golgi apparatus, equivalent to the trans-Golgi network. After osmium impregnation all dictyosomal cisternae are filled with electron-dense deposits that are also found in thick-membrane cisternae and in some alveolar sacs. The presence of TPPase and NDPase activities in the alveolar sacs may be related to glycosyltransferase activities, as proved in other cells. Our new results suggest that the previous detection of AcPase activity in the alveolar sacs was an artefact.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7