Improved method for fatty acid analysis in herbage based on direct transesterification followed by solid-phase extraction

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Alves, SP (Autor) Outra; A. Cabrita (Autor) FCUP; Antonio J M Fonseca (Autor) ICBAS; Bessa, RJB (Autor) Outra

Revista

Título: Journal of Chromatography A

Vol. 1209

Páginas: 212-219

ISSN: 0021-9673

Editora: Elsevier

Indexação

ISI Web of Knowledge - 39 Citações

Scopus - 41 Citações

Classificação Científica

CORDIS: Ciências Naturais > Ciências agrárias > Zootecnia

FOS: Ciências agrárias > Ciência animal e dos lacticínios

Outras Informações

ID Authenticus: P-003-VE9

DOI: 10.1016/j.chroma.2008.08.114

Abstract (EN): Direct transesterification (DT) and solvent extraction with acid or basic derivatization procedure for fatty acid (FA) analysis in herbage were compared. The highest total FA, alpha-linolenic and linoleic acid contents were obtained with DT. However, DT also produced the highest amount of interfering compounds, identified as phytadienes and sugar derivative products, which may co-elute with FA. An additional step based on solid-phase extraction to produce clean samples was proposed. This procedure is simple and gives good recoveries for the FA fortified samples. Additionally, structural characterization of 16:1 trans-3 was conducted by covalent adduct chemical ionization tandem mass spectrometry.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8