A New Pathway of CD5 Glycoprotein-mediated T Cell Inhibition Dependent on Inhibitory Phosphorylation of Fyn Kinase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Bamberger, M (Autor) Outra; Santos, AM (Autor) Outra; Goncalves, CM (Autor) Outra; Oliveira, MI (Autor) Outra; James, JR (Autor) Outra; Moreira, A (Autor) REIT; Lozano, F (Autor) Outra; Davis, SJ (Autor) Outra; Alexandre Carmo (Autor) ICBAS

Revista

Título: Journal of Biological Chemistry

Vol. 286

Páginas: 30324-30336

ISSN: 0021-9258

Editora: Elsevier

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-002-MMW

DOI: 10.1074/jbc.m111.230102

Abstract (EN): Triggering of the T cell receptor initiates a signaling cascade resulting in the activation of the T cell. These signals are integrated alongside those resulting from the triggering of other receptors whose function is to modulate the overall response. CD5 is an immunotyrosine-based inhibition motif-bearing receptor that antagonizes the overt T cell receptor activation response by recruiting inhibitory intracellular mediators such as SHP-1, RasGAP, or Cbl. We now propose that the inhibitory effects of CD5 are also mediated by a parallel pathway that functions at the level of inhibition of Fyn, a kinase generally associated with T cell receptor-mediated activation. After CD5 ligation, phosphorylation of the negative regulatory tyrosine (Tyr(531)) of Fyn increases, and this correlates with a substantial reduction in the kinase activity of Fyn and a profound inhibition of ZAP-70 activation. The effect requires the last 23 amino acids of the cytoplasmic domain of the receptor, strongly implying the involvement of a new CD5-interacting signaling or adaptor protein. Furthermore, we show that upon CD5 ligation there is a profound shift in its distribution from the bulk fluid phase to the lipid raft environment, where it associates with Fyn, Lck, and PAG. We suggest that the relocation of CD5, which we also show is capable of forming homodimers, to the proximity of raft-resident molecules enables CD5 to inhibit membrane proximal signaling by controlling the phosphorylation and activity of Fyn, possibly by interfering with the disassembly of C-terminal Src kinase (Csk)-PAG-Fyn complexes during T cell activation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13