Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Goncalves, CM (Autor) Outra; Castro, MAA (Autor) Outra; Henriques, T (Autor) Outra; Oliveira, MI (Autor) Outra; Pinheiro, HC (Autor) Outra; Oliveira C (Autor) FMUP; Sreenu, VB (Autor) Outra; Evans, EJ (Autor) Outra; Davis, SJ (Autor) Outra; Moreira, A (Autor) REIT; Alexandre Carmo (Autor) ICBAS

Revista

Título: Molecular Immunology

Vol. 46

Páginas: 2585-2596

ISSN: 0161-5890

Editora: Elsevier

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-003-H8H

DOI: 10.1016/j.molimm.2009.05.006

Abstract (EN): Glycoproteins of the scavenger receptor cysteine-rich (SRCR) superfamily contain one or more protein modules homologous to the membrane-distal domain of macrophage scavenger receptor I. These domains can be found in the extracellular regions of membrane proteins and in secreted glycoproteins, from the most primitive species to vertebrates. A systematic, bioinformatics-based search for putative human proteins related to the forty-seven known human group B SRCR domains identified a new family member that we have called Soluble Scavenger with 5 Domains (SSc5D). SSc5D is a new soluble protein whose expression is restricted to monocytes/macrophages and T-lymphocytes, and is particularly enriched in the placenta. The gene encoding SSc5D spans 30 kb of genomic DNA, and contains fourteen exons producing a 4.8 kb-long mRNA. The mature polypeptide is predicted to consist of 1573 amino acids comprising, towards the N-terminus, five very similar SRCR domains that are highly conserved among non-marsupial mammals, and a large (>250 nm), very heavily glycosylated, mucin-like sequence towards the C-terminus. Each of the SRCR domains is encoded by a single exon, and contains eight cysteine residues, as observed for all other group B SRCR domains. A shorter isoform encoded by a weakly expressed, alternatively spliced transcript, which lacks the mucin-like C-terminal region, was also identified. It seems likely that SSc5D has a role at the interface between adaptive and innate immunity, or in placental function.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12