Polypyrimidine tract binding protein modulates efficiency of polyadenylation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Castelo Branco, P (Autor) Outra; Furger, A (Autor) Outra; Wollerton, M (Autor) Outra; Smith, C (Autor) Outra; Moreira, A (Autor) REIT; Proudfoot, N (Autor) Outra

Revista

Título: Molecular and Cellular Biology

Vol. 24

Páginas: 4174-4183

ISSN: 0270-7306

Editora: American Society for Microbiology

Indexação

ISI Web of Knowledge - 119 Citações

Scopus - 104 Citações

Outras Informações

ID Authenticus: P-000-ANW

DOI: 10.1128/mcb.24.10.4174-4183.2004

Abstract (EN): Polypyrimidine tract binding protein (PTB) is a major hnRNP protein with multiple roles in mRNA metabolism, including regulation of alternative splicing and internal ribosome entry site-driven translation. We show here that a fourfold overexpression of PTB results in a 75% reduction of mRNA levels produced from transfected gene constructs with different polyadenylation signals (pA signals). This effect is due to the reduced efficiency of mRNA 3' end cleavage, and in vitro analysis reveals that PTB competes with CstF for recognition of the pA signal's pyrimidine-rich downstream sequence element. This may be analogous to its role in alternative splicing, where PTB competes with U2AF for binding to pyrimidine-rich intronic sequences. The pA signal of the C2 complement gene unusually possesses a PTB-dependent upstream sequence, so that knockdown of PTB expression by RNA interference reduces C2 mRNA expression even though PTB overexpression still inhibits polyadenylation. Consequently, we show that PTB can act as a regulator of mRNA expression through both its negative and positive effects on mRNA 3' end processing.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10