THE SPINDLE IS REQUIRED FOR THE PROCESS OF SISTER CHROMATID SEPARATION IN DROSOPHILA NEUROBLASTS

• Tipo: Artigo em Revista Científica Internacional
• Data: 1991

Autores

GONZALEZ, C (Autor) Outra; JIMENEZ, JC (Autor) Outra; RIPOLL, P (Autor) Outra; Sunkel, CE (Autor) Outra

Revista

Título: Experimental Cell Research

Vol. 192

Páginas: 10-15

ISSN: 0014-4827

Editora: Elsevier

Indexação

ISI Web of Knowledge - 39 Citações

Scopus - 31 Citações

Outras Informações

ID Authenticus: P-001-R3Y

DOI: 10.1016/0014-4827(91)90150-s

Abstract (EN): We have studied two aspects of the process of sister chromatid separation in the Drosophila melanogaster neuroblasts. First, we analyzed the requirement of a functional spindle for sister chromatid separation to take place using microtubule depolymerizing drugs such as colchicine or a reversible analogue (MTC). Incubation of this tissue in colchicine causes the cells to block irreversibly at metaphase and no significant levels of sister chromatid separation were observed even after long periods of incubation. Exposure of neuroblasts to MTC also causes cells to block at metaphase, but after reversion most of the cells enter anaphase and are thus able to complete sister chromatid separation. These results imply that a functional spindle is required for sister chromatid separation. Second, we studied the role of heterochromatin during chromatid pairing and subsequent separation in chromosomes which carry either one or two extra pieces of heterochromatin. The results indicate that sister chromatids establish strong pairing along the translocated heterochromatin. During the early stages of anaphase, these chromosomes separate first the centromeric region and later the regions bearing extra heterochromatin. These results indicate that constitutive heterochromatin plays an important role for sister chromatid pairing and might be involved in the process of separation. © 1991 Academic Press, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6