The Drosophila ¿-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Colombie, N (Autor) Outra; Verollet, C (Autor) Outra; Sampaio, P (Autor) Outra; Moisand, A (Autor) Outra; Sunkel, CE (Autor) ICBAS; Bourbon, HM (Autor) Outra; Wright, M (Autor) Outra; Raynaud Messina, B (Autor) Outra

Revista

Título: Molecular Biology of the Cell

Vol. 17

Páginas: 272-282

ISSN: 1059-1524

Editora: American Society for Cell Biology

Indexação

Scopus - 37 Citações

Outras Informações

ID Authenticus: P-00F-H9H

DOI: 10.1091/mbc.e05-08-0722

Abstract (EN): ¿-Tubulin, a protein critical for microtubule assembly, functions within multiprotein complexes. However, little is known about the respective role of ¿-tubulin partners in metazoans. For the first time in a multicellular organism, we have investigated the function of Dgrip84, the Drosophila orthologue of the Saccharomyces cerevisiae ¿-tubulin-associated protein Spc97p. Mutant analysis shows that Dgrip84 is essential for viability. Its depletion promotes a moderate increase in the mitotic index, correlated with the appearance of monopolar or unpolarized spindles, impairment of centrosome maturation, and increase of polyploid nuclei. This in vivo study is strengthened by an RNA interference approach in cultured S2 cells. Electron microscopy analysis suggests that monopolar spindles might result from a failure of centrosome separation and an unusual microtubule assembly pathway via centriolar triplets. Moreover, we point to an involvement of Dgrip84 in the spindle checkpoint regulation and in the maintenance of interphase microtubule dynamics. Dgrip84 also seems essential for male meiosis, ensuring spindle bipolarity and correct completion of cytokinesis. These data sustain that Dgrip84 is required in some aspects of microtubule dynamics and organization both in interphase and mitosis. The nature of a minimal ¿-tubulin complex necessary for proper microtubule organization in the metazoans is discussed. © 2005 by The American Society for Cell Biology.

Idioma: Inglês

Tipo (Avaliação Docente): Científica