RNA polymerase II kinetics in polo polyadenylation signal selection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Pinto, PAB (Autor) Outra; Henriques, T (Autor) Outra; Freitas, MO (Autor) Outra; Martins, T (Autor) Outra; Domingues, RG (Autor) Outra; Wyrzykowska, PS (Autor) Outra; Coelho, PA (Autor) Outra; Alexandre Carmo (Autor) ICBAS; Sunkel, CE (Autor) ICBAS; Proudfoot, NJ (Autor) Outra; Moreira, A (Autor) REIT

Revista

Título: EMBO Journal

Vol. 30

Páginas: 2431-2444

ISSN: 0261-4189

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 73 Citações

Scopus - 88 Citações

Outras Informações

ID Authenticus: P-002-QEV

DOI: 10.1038/emboj.2011.156

Abstract (EN): Regulated alternative polyadenylation is an important feature of gene expression, but how gene transcription rate affects this process remains to be investigated. polo is a cell-cycle gene that uses two poly(A) signals in the 30 untranslated region (UTR) to produce alternative messenger RNAs that differ in their 3'UTR length. Using a mutant Drosophila strain that has a lower transcriptional elongation rate, we show that transcription kinetics can determine alternative poly(A) site selection. The physiological consequences of incorrect polo poly(A) site choice are of vital importance; transgenic flies lacking the distal poly(A) signal cannot produce the longer transcript and die at the pupa stage due to a failure in the proliferation of the precursor cells of the abdomen, the histoblasts. This is due to the low translation efficiency of the shorter transcript produced by proximal poly(A) site usage. Our results show that correct polo poly(A) site selection functions to provide the correct levels of protein expression necessary for histoblast proliferation, and that the kinetics of RNA polymerase II have an important role in the mechanism of alternative polyadenylation. The EMBO Journal (2011) 30, 2431-2444. doi: 10.1038/emboj.2011.156; Published online 20 May 2011

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14