Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Martins, T (Autor) Outra; Maia, AF (Autor) Outra; Steffensen, S (Autor) Outra; Sunkel, CE (Autor) ICBAS

Revista

Título: EMBO Journal

Vol. 28

Páginas: 234-247

ISSN: 0261-4189

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 41 Citações

Outras Informações

ID Authenticus: P-003-N7X

DOI: 10.1038/emboj.2008.283

Abstract (EN): Sgt1 was described previously in yeast and humans to be a Hsp90 co-chaperone and required for kinetochore assembly. We have identified a mutant allele of Sgt1 in Drosophila and characterized its function. Mutations in sgt1 do not affect overall kinetochore assembly or spindle assembly checkpoint. sgt1 mutant cells enter less frequently into mitosis and arrest in a prometaphase-like state. Mutations in sgt1 severely compromise the organization and function of the mitotic apparatus. In these cells, centrioles replicate but centrosomes fail to mature, and pericentriolar material components do not localize normally resulting in highly abnormal spindles. Interestingly, a similar phenotype was described previously in Hsp90 mutant cells and correlated with a decrease in Polo protein levels. In sgt1 mutant neuroblasts, we also observe a decrease in overall levels of Polo. Overexpression of the kinase results in a substantial rescue of the centrosome defects; most cells form normal bipolar spindles and progress through mitosis normally. Taken together, these findings suggest that Sgt1 is involved in the stabilization of Polo allowing normal centrosome maturation, entry and progression though mitosis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14