Drosophila CENP-C is essential for centromere identity

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Orr, B (Autor) Outra; Sunkel, CE (Autor) ICBAS

Revista

Título: Chromosoma

Vol. 120

Páginas: 83-96

ISSN: 0009-5915

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 21 Citações

Outras Informações

ID Authenticus: P-002-VEK

DOI: 10.1007/s00412-010-0293-6

Abstract (EN): Centromeres are specialized chromosomal domains that direct mitotic kinetochore assembly and are defined by the presence of CENP-A (CID in Drosophila) and CENP-C. While the role of CENP-A appears to be highly conserved, functional studies in different organisms suggest that the precise role of CENP-C in kinetochore assembly is still under debate. Previous studies in vertebrate cells have shown that CENP-C inactivation causes mitotic delay, chromosome missegregation, and apoptosis; however, in Drosophila, the role of CENP-C is not well-defined. We have used RNA interference depletion in S2 cells to address this question and we find that depletion of CENP-C causes a kinetochore null phenotype, and consequently, the spindle checkpoint, kinetochore-microtubule interactions, and spindle size are severely misregulated. Importantly, we show that CENP-C is required for centromere identity as CID, MEI-S332, and chromosomal passenger proteins fail to localize in CENP-C depleted cells, suggesting a tight communication between the inner kinetochore proteins and centromeres. We suggest that CENP-C might fulfill the structural roles of the human centromere-associated proteins not identified in Drosophila.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14