GAMMA-TUBULIN IS REQUIRED FOR THE STRUCTURE AND FUNCTION OF THE MICROTUBULE-ORGANIZING CENTER IN DROSOPHILA NEUROBLASTS

• Tipo: Artigo em Revista Científica Internacional
• Data: 1995

Autores

Sunkel, CE (Autor) ICBAS; GOMES, R (Autor) Outra; SAMPAIO, P (Autor) Outra; PERDIGAO, J (Autor) Outra; GONZALEZ, C (Autor) Outra

Revista

Título: EMBO Journal

Vol. 14

Páginas: 28-36

ISSN: 0261-4189

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 164 Citações

Scopus - 159 Citações

Outras Informações

ID Authenticus: P-001-HDV

Abstract (EN): We report that in Drosophila, gamma-tubulin is required for the structure as well as the function of microtubule organizing centres (MTOCs). This conclusion is based on the identification and phenotypic characterization of a mutant allele of the gamma-tubulin gene located at region 23C of the polytene chromosome map. This mutation, which we have called gamma-tub23C(PI), is caused by the insertion of a P-element within the 5' untranslated leader of the gamma-tubulin transcript. Northern and Western analyses show that gamma-tub23C(PI) is either a null or a very severe hypomorph as no gamma-tubulin mRNA or protein can be detected in mutant individuals. Visualization of DNA, MTOCs and microtubules by confocal laser scanning microscopy of cells from individuals homozygous for gamma-tub23C(PI) reveals a series of phenotypic abnormalities. Some of these are similar to those observed after disruption of gamma-tubulin function in other organisms, including mitotic arrest and a dramatic decrease in the number of microtubules, but, in addition, we have observed that mutation in this gene also results in highly abnormal MTOCs which show a variety of shapes and sizes which we never observed in wild type cells. These results show that gamma-tubulin is required for both structural and functional roles in the MTOCs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9