Experimental vitrification of human compacted morulae and early blastocysts using fine diameter plastic micropipettes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Cremades, N (Autor) Outra; sousa, m (Autor) ICBAS; Silva, J (Autor) Outra; Viana, P (Autor) Outra; Sousa, S (Autor) Outra; Oliveira, C (Autor) Outra; da Silva, JT (Autor) Outra; barros, a (Autor) FMUP

Revista

Título: Human Reproduction

Vol. 19

Páginas: 300-305

ISSN: 0268-1161

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 46 Citações

Outras Informações

ID Authenticus: P-000-BQX

DOI: 10.1093/humrep/deh059

Abstract (EN): BACKGROUND: Vitrification of human blastocysts has been successfully applied using grids, straws and cryoloops. We assessed the survival rate of human compacted morulae and early blastocysts vitrified in pipette tips with a smaller inner diameter and solution volume than the previously described open pulled straw (OPS) method. METHODS: Excess day 5 human embryos (n = 63) were experimentally vitrified in vessels. Embryos were incubated at 37degreesC with sperm preparation medium (SPM) for 1 min, SPM + 7.5% ethylene glycol (EG)/dimethylsulphoxide (DMSO) for 3 min, and SPM + 16.5% EG + 16.5% DMSO + 0.67 mol/l sucrose for 25 s. They were then aspirated (0.5 mul) into a plastic micropipette tip (0.36 mm inner diameter), exposed to liquid nitrogen (LN2) vapour for 2 min before being placed into a pre-cooled cryotube, which was then closed and plunged into LN2. Embryos were warmed and diluted using 0.33 mol/l and 0.2 mol/l sucrose. RESULTS: The survival rate for compacted morulae was 73% (22/30) and 82% (27/33) for early blastocysts. CONCLUSIONS: The survival rates of human compacted morulae and early blastocysts after vitrification with this simple technique are similar to those reported in the literature achieved by slow cooling and other vitrification protocols.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6