Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Cremades, N (Autor) Outra; sousa, m (Autor) ICBAS; Bernabeu, R (Autor) Outra; barros, a (Autor) FMUP

Revista

Título: Human Reproduction

Vol. 16

Páginas: 1938-1944

ISSN: 0268-1161

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 45 Citações

Scopus - 55 Citações

Outras Informações

ID Authenticus: P-000-TD3

DOI: 10.1093/humrep/16.9.1938

Abstract (EN): BACKGROUND: Round spermatid injections are associated with disappointing clinical outcomes, and although these cells have been shown to mature into late spermatids in vitro, the developmental potential of such gametes remains to be demonstrated. METHODS: Round spermatids were isolated from 12 testicle samples of patients with obstructive azoospermia, hypoplasia, complete maturation arrest, and incomplete Sertoli cell-only syndrome. They were cultured for 7 days at 32 degreesC, 5% CO2 in air, in microdrops of Vero cell-conditioned medium containing 10% synthetic serum substitute. RESULTS: From the 238 round spermatids cultured, 25.2% attained the elongating and 5.5% the elongated spermatid stage (3-4 days per step). Relatively higher maturation rates were found in cases with obstructive azoospermia, but differences were significant only for elongated spermatids (9.3%). No differences were found in maturation rates between cases with non-obstructive azoospermia (4.3% of elongated spermatids). Experimental microinjections with elongating and elongated spermatids revealed a low fertilization rate (40.9%) but a normal blastocyst formation rate (60%). CONCLUSIONS: Late spermatids resulting from in-vitro culture of round spermatids in conditioned medium, either in controls in cases with a spermiogenetic block, appeared able to successfully fertilize the human oocyte and elicit normal embryo development.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7