Phosphatidylserine translocation in human spermatozoa from impaired spermatogenesis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Almeida C (Autor) FMUP; sousa, m (Autor) ICBAS; barros, a (Autor) FMUP

Revista

Título: Reproductive BioMedicine Online

Vol. 19

Páginas: 770-777

ISSN: 1472-6483

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 18 Citações

Outras Informações

ID Authenticus: P-003-E7A

DOI: 10.1016/j.rbmo.2009.10.002

Abstract (EN): The aim of the present study was to evaluate phosphatidylserine translocation in specific patient groups and compare the rates of apoptosis between ejaculated and testicular spermatozoa. Fifty-six patients undergoing infertility treatments were included in the present study. Semen samples (n = 37) were obtained from cases with normozoospermia (it = 9) and abnormal semen parameters (n = 28). Testicular biopsy was performed in 19 patients, eight with obstructive and six with non-obstructive (hypospermatogenesis) azoospermia, and in five patients without azoospermia (anejaculation and oligozoospermia). Phosphatidylserine externalization was assessed using annexin-V binding and fluorescence microscopy, and propidium iodide exclusion tests were used to distinguish live from dead cells. In semen, oligoasthenoteratozoospermia showed significantly increased rates of sperm apoptosis (60.3 +/- 12.9) than normozoospermia (47.5 +/- 10.2). In testis, hypospermatogenesis (63.3 +/- 10.3) and obstructive azoospermia (63.6 +/- 15.1) showed significantly increased rates of sperm apoptosis than non-azoospermic patients (49.6 +/- 25.5). Comparisons between semen and testis showed that oligozoospermia had significantly higher rates of sperm apoptosis in semen (57.9 +/- 11.9) than in testis (29.4 +/- 1.1). The results suggest the presence of a post-testicular apoptotic induction factor and the potential beneficial use of testicular spermatozoa in clinical treatments.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8