Effect of insulin deprivation on metabolism and metabolism-associated gene transcript levels of in vitro cultured human Sertoli cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Oliveira, PF (Autor) Outra; Alves, MG (Autor) Outra; Rato, L (Autor) Outra; Laurentino, S (Autor) Outra; Silva, J (Autor) Outra; Sa, R (Autor) ICBAS; barros, a (Autor) FMUP; sousa, m (Autor) ICBAS; Carvalho, RA (Autor) Outra; cavaco, je (Autor) Outra; Socorro, S (Autor) Outra

Revista

Título: Biochimica et Biophysica Acta - General Subjects

Vol. 1820

Páginas: 84-89

ISSN: 0304-4165

Editora: Elsevier

Indexação

ISI Web of Knowledge - 105 Citações

Scopus - 116 Citações

Outras Informações

ID Authenticus: P-002-DER

DOI: 10.1016/j.bbagen.2011.11.006

Abstract (EN): Background: Sertoli cells metabolize glucose producing lactate for developing germ cells. As insulin regulates glucose uptake and its disturbance/insensitivity is associated with diabetes mellitus, we aimed to determine the effect of insulin deprivation in human Sertoli cell (hSC) metabolism and metabolism-associated gene expression. Methods: hSC-enriched primary cultures were maintained in the absence/presence of insulin and metabolite variations were determined by H-1-NMR. mRNA expression levels of glucose transporters (GLUT1, GLUT3), lactate dehydrogenase (LDHA) and monocarboxylate transporter (MCT4) were determined by RT-PCR. Results: Insulin deprivation resulted in decreased lactate production and in decrease of glucose consumption that was completely reverted after 6 h. Cells of both groups consumed similar amounts of glucose. In insulin-deprived cells, transcript levels of genes associated to lactate metabolism (LDHA and MCT4) were decreased. Transcript levels of genes involved in glucose uptake exhibited a divergent variation: GLUT3 levels were decreased while GLUT1 levels increased. Insulin-deprived hSCs presented: 1) altered glucose consumption and lactate secretion; 2) altered expression of metabolism-associated genes involved in lactate production and export; 3) an adaptation of glucose uptake by modulating the expression of GLUT1 and GLUT3. General significance: This is the first report regarding the effect of insulin-deprivation on hSC metabolism.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6