STARFISH SPERM-OOCYTE JELLY BINDING TRIGGERS FUNCTIONAL-CHANGES IN CORTICAL GRANULES - A STUDY USING ACID-PHOSPHATASE AND RUTHENIUM RED ULTRASTRUCTURAL HISTOCHEMISTRY

• Tipo: Artigo em Revista Científica Internacional
• Data: 1989

Autores

sousa, m (Autor) ICBAS; AZEVEDO, C (Autor) Outra

Revista

Título: Histochemistry

Vol. 90

Páginas: 353-357

ISSN: 0301-5564

Editora: Springer

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-001-SXC

DOI: 10.1007/bf00508312

Abstract (EN): Starfish oocytes were examined before fertilization, immediately after insemination, and during the cortical reaction by means of acid phosphatase and ruthenium red ultrastructural histochemistry. Oocyte cortical granules are composed of a lamellar body and a surrounding matrix which is subdivided into dense and light portions. In unfertilized oocytes cortical granules are not stained by ruthenium red but show a weak acid phosphatase activity in the light portion of the granule matrix. Immediately after the adhesion of the spermatozoon to the oocyte jelly coat, the light matrix portion of cortical granules appears stained by ruthenium red and shows a strong acid phosphatase activity. During the cortical reaction, cortical granules are released into the perivitelline space and the lamellar body, surrounded by the stained matrix, fuses with the fertilization envelope. Our data suggest that membrane permeability changes and enzyme activation occur in the egg when the spermatozoon binds to the oocyte jelly coat.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5