Effect of microsatellite selection on individual and population genetic inferences: an empirical study using cross-specific and species-specific amplifications

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Queiros, J (Autor) Outra; Godinho, R (Autor) Outra; Lopes, S (Autor) Outra; Gortazar, C (Autor) Outra; de la Fuente, J (Autor) Outra; Alves, PC (Autor) FCUP

Revista

Título: Molecular Ecology Resources

Vol. 15

Páginas: 747-760

ISSN: 1755-098X

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 52 Citações

Scopus - 52 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-00G-C10

DOI: 10.1111/1755-0998.12349

Abstract (EN): Although whole-genome sequencing is becoming more accessible and feasible for nonmodel organisms, microsatellites have remained the markers of choice for various population and conservation genetic studies. However, the criteria for choosing microsatellites are still controversial due to ascertainment bias that may be introduced into the genetic inference. An empirical study of red deer (Cervus elaphus) populations, in which cross-specific and species-specific microsatellites developed through pyrosequencing of enriched libraries, was performed for this study. Two different strategies were used to select the species-specific panels: randomly vs. highly polymorphic markers. The results suggest that reliable and accurate estimations of genetic diversity can be obtained using random microsatellites distributed throughout the genome. In addition, the results reinforce previous evidence that selecting the most polymorphic markers leads to an ascertainment bias in estimates of genetic diversity, when compared with randomly selected microsatellites. Analyses of population differentiation and clustering seem less influenced by the approach of microsatellite selection, whereas assigning individuals to populations might be affected by a random selection of a small number of microsatellites. Individual multilocus heterozygosity measures produced various discordant results, which in turn had impacts on the heterozygosity-fitness correlation test. Finally, we argue that picking the appropriate microsatellite set should primarily take into account the ecological and evolutionary questions studied. Selecting the most polymorphic markers will generally overestimate genetic diversity parameters, leading to misinterpretations of the real genetic diversity, which is particularly important in managed and threatened populations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14