Xanthohumol impairs glucose uptake by a human first-trimester extravillous trophoblast cell line (HTR-8/SVneo cells) and impacts the process of placentation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Ana Correia Branco (Autor) Outra; Claudia F Azevedo (Autor) Outra; Joao R Araujo (Autor) Outra; Joao T Guimaraes (Autor) Outra; Ana Faria (Autor) FCNAUP; Elisa Keating (Autor) Outra; Fatima Martel (Autor) Outra

Revista

Título: Molecular Human Reproduction

Vol. 21

Páginas: 803-815

ISSN: 1360-9947

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 20 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-00G-QGF

DOI: 10.1093/molehr/gav043

Abstract (EN): In this study, we aimed to investigate modulation of glucose uptake by the HTR-8/SVneo human first-trimester extravillous trophoblast cell line by a series of compounds and to study its consequences upon cell proliferation, viability and migration. We observed that uptake of H-3-deoxy-D-glucose (H-3-DG; 10 nM) was time-dependent, saturable, inhibited by cytochalasin B (50 and 100 mu M), phloretin (0.5 mM) and phloridzin (1 mM), insulin-insensitive and sodium-independent. In the short term (30 min), neither 5-HT (100-1000 mu M), melatonin (10 nM) nor the drugs of abuse ethanol (100 mu M), nicotine (100 mu M), cocaine (25 mu M), amphetamine (10-25 mu M) and 3,4-methylene-dioxy-N-methamphetamine (10 mu M) affected 3H-DG uptake, while dexamethasone (100-1000 mu M), fluoxetine (100-300 mu M), quercetin, epigallocatechin-3-gallate (30-1000 mu M), xanthohumol (XH) and resveratrol (1-500 mu M) decreased it. XH was the most potent inhibitor [IC50 = 3.55 (1.37-9.20) mu M] of H-3-DG uptake, behaving as a non-competitive inhibitor of 3H-DG uptake, both after short-and long-term (24 h) treatment. The effect of XH(5 mu M; 24 h) upon H-3-DG uptake involved mammalian target of rapamycin, tyrosine kinases and c-Jun N-terminal kinases intracellular pathways. Moreover, XH appeared to decrease cellular uptake of lactate due to inhibition of the monocarboxylate transporter 1. Additionally, XH (24 h; 5 mu M) decreased cell viability, proliferation, culture growth and migration. The effects of XH upon cell viability and culture growth, but not the antimigratory effect, were mimicked by low extracellular glucose conditions and reversed by high extracellular glucose conditions. We thus suggest that XH, by inhibiting glucose cellular uptake and impairing HTR-8/SVneo cell viability and proliferation, may have a deleterious impact in the process of placentation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13