Equisetum arvense hydromethanolic extracts in bone tissue regeneration: in vitro osteoblastic modulation and antibacterial activity

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

C. Bessa Pereira (Autor) Outra; Gomes PS (Autor) FMDUP; João Costa-Rodrigues (Autor) FMDUP; R. Almeida Palmas (Autor) Outra; L. Vieira (Autor) ICBAS; Ferraz, MP (Autor) Outra; M. A. Lopes (Autor) FEUP; M.H. Fernandes (Autor) FMDUP

Revista

Título: Cell Proliferation

Vol. 45 Nº 4

Páginas: 386-396

ISSN: 0960-7722

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 34 Citações

Pubmed / Medline

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências médicas e da saúde

Outras Informações

ID Authenticus: P-002-7RE

DOI: 10.1111/j.1365-2184.2012.00826.x

Abstract (EN): Objectives E quisetum arvense preparations have long been used to promote bone healing. The aim of this work was to evaluate osteogenic and antibacterial effects of E . arvense hydromethanolic extracts. Materials and methods Dried aerial components of E . arvense were extracted using a mixture of methanol:water (1:1), for 26 days, yielding three extracts that were tested (10–1000 μg/ml) in human osteoblastic cells: E1, E2 and EM (a mixture of E1 and E2, 1:1). Cell cultures, performed on cell culture plates or over hydroxyapatite (HA) substrates, were assessed for osteoblastic markers. In addition, effects of the extracts on S taphylococcus aureus were addressed. Results Solution E1 caused increased viability/proliferation and ALP activity at 50–500 μg/ml, and deleterious effects at levels ≥1000 μg/ml. E2 inhibited cell proliferation at levels ≥500 μg/ml. EM presented a profile between those observed with E1 and E2. In addition, E1, E2 and EM, 10–1000 μg/ml, inhibited expansion of S . aureus. Furthermore, E1, tested in HA substrates colonized with osteoblastic cells, causing increase in cell population growth (10–100 μg/ml). E1 also exhibited antibacterial activity against S . aureus cultured over HA. Conclusions Results showed that E . arvense extracts elicited inductive effects on human osteoblasts while inhibiting activity of S . aureus, suggesting a potentially interesting profile regarding bone regeneration strategies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica