Diversity and biofilm-production ability among isolates of Escherichia coli phylogroup D belonging to ST69, ST393 and ST405 clonal groups

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Angela Novais (Autor) Outra; Claudia Vuotto (Autor) Outra; Joao Pires (Autor) Outra; Carolina Montenegro (Autor) Outra; Gianfranco Donelli (Autor) Outra; Teresa M Coque (Autor) Outra; Luisa Peixe (Autor) FFUP

Revista

Título: BMC Microbiology

Vol. 13

Página Final: 144

ISSN: 1471-2180

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 25 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-006-A0S

DOI: 10.1186/1471-2180-13-144

Abstract (EN): Background: Phylogenetic group D Escherichia coli clones (ST69, ST393, ST405) are increasingly reported as multidrug resistant strains causing extra-intestinal infections. We aim to characterize inter- and intraclonal diversity of a broad sample (isolates from different geographic locations and origins with variable antibiotic resistance profiles, 1980-2010) and their ability to adhere and form biofilm by both a modified quantitative biofilm producing assay and Field Emission Scanning Electron Microscopy (FESEM). Results: High virulence scores were observed among ST69 (median 14/range 9-15) and ST393 (median 14/range 8-15) clones, particularly enriched in pap alleles, iha, kpsMTII-K5 and ompT, in contrast with ST405 (median 6/range 2-14) isolates, exhibiting frequently fyuA, malX and traT. All ST69 and ST393 and only two ST405 isolates were classified as ExPEC. Biofilm production was detected in two non-clinical ST69 and three ST393 isolates from different origins showing variable virulence profiles. Within each clonal group, and despite the high diversity of PFGE-types observed, isolates from different countries and recovered over large periods of time were clustered in a few groups sharing common virulence gene profiles among ST69 (n = 10 isolates) and ST393 (n = 9 isolates) (fimH-iha-iutA-kpsMTII-K5-(traT)-sat-(ompT)-papA-papEF-papGII-papC) or ST405 (n = 6 isolates) (fimH-traT-fyuA-malX). Conclusions: This study highlights the circulation of highly transmissible ST69, ST393 and ST405 variants among different settings. Biofilm production seems not to be directly correlated with their epidemiological success.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9