A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Sonia Soares (Autor) Outra; Joana S Amaral (Autor) Outra; Beatriz B P P Oliveira (Autor) FFUP; Isabel Mafra (Autor) FFUP

Revista

Título: Meat Science

Vol. 94

Páginas: 115-120

ISSN: 0309-1740

Editora: Elsevier

Indexação

ISI Web of Knowledge - 69 Citações

Scopus - 105 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-005-1F9

DOI: 10.1016/j.meatsci.2012.12.012

Abstract (EN): Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye was proposed for the quantitative detection of pork meat in processed meat products. For the development of the method, binary meat mixtures containing known amounts of pork meat in poultry meat were used to obtain a normalised calibration model from 0.1 to 25% with high linear correlation and PCR efficiency. The method revealed high specificity by melting curve analysis, being successfully validated through its application to blind meat mixtures, which confirmed its adequacy for pork meat determination. The fully applicability of the method was further demonstrated in commercial meat products, allowing verification of labelling compliance and identification of meat species in processed foods.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6