A yeast model of the Parkinson's disease-associated protein Parkin

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Clara Pereira (Autor) Outra; Vitor Costa (Autor) Outra; Miguel Martins, LM (Autor) Outra; Lucilia Saraiva (Autor) FFUP

Revista

Título: Experimental Cell Research

Vol. 333

Páginas: 73-79

ISSN: 0014-4827

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 20 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-00A-A8P

DOI: 10.1016/j.yexcr.2015.02.018

Abstract (EN): Mutations in Parkin, an E3 ubiquitin ligase, are associated to autosomal recessive Parkinson's disease (PD). Parkin has been mainly implicated, along with Pink1, in mitochondrial autophagy in response to stress. In this study, a yeast model was developed to analyse the biological function of human Parkin. We observed that Parkin increases yeast chronological lifespan and oxidative stress resistance, through a mitochondrial-dependent pathway. Moreover, in response to H2O2, Parkin translocate to mitochondria, leading to a higher mitochondrial degradation. Parkin-induced H2O2 resistance is dependent on the autophagic pathway and on the mitochondrial protein Por1 p. Although expression of Pink1 induces an H2O2 resistance phenotype similar to Parkin, co-expression of both proteins does not result in a synergistic effect. Concerning H2O2 resistance, this may indicate that these two proteins independently affect the same pathway. Altogether, this work establishes a yeast model for Parkin, which may provide new insights on Parkin function and potential mechanisms of pathogenicity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7