Characterization and evaluation of BNIPDaoct-loaded PLGA nanoparticles for visceral leishmaniasis: in vitro and in vivo studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Sofia A C Costa Lima (Autor) Outra; Mariana Resende (Autor) Outra; Ricardo Silvestre (Autor) Outra; Joana Tavares (Autor) Outra; Ali Ouaissi (Autor) Outra; Paul Kong Thoo Lin (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP

Revista

Título: Nanomedicine

Vol. 7

Páginas: 1839-1849

ISSN: 1743-5889

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 31 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Nanotecnologia

Outras Informações

ID Authenticus: P-002-2RG

DOI: 10.2217/nnm.12.74

Abstract (EN): Objective: To overcome the limitation of bisnaphthalimidopropyldiaaminooctane (BNIPDaoct) low physiological solubility and potentially increase its efficiency against visceral leishmaniasis (VL), a delivery system based on poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles was developed. Materials & methods: BNIPDaoct PLGA nanoparticles were prepared by nanoprecipitation and characterized. Anti-Leishmania activity was evaluated using in vitro and in vivo VL infection models. Results: BNIPDaoct PLGA nanoparticles were successfully produced and were sized at 156.0 +/- 2.8 nm with an encapsulation efficiency of approximately 85%. The PLGA nanoparticles reduced BNIPDaoct cellular toxicity, retained its in vitro anti-leishmanial activity and led to a significant reduction (similar to 80%) in the parasite burden in the infected mice spleen when compared with the free drug or amphotericin B. In the liver the effect was less pronounced, with a 30-50% reduction observed between the nanoformulation and the BNIIPDaoct per se or the amphotericin B, respectively. Conclusion: PLGA nanoparticles provide controlled and effective delivery of BNIPDaoct for treatment of VL. Original submitted 23 December 2012; Revised submitted 5 April 2012; Published online 20 July 2012

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11