Endocytosis inhibition during H2O2-induced apoptosis in yeast

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Clara Pereira (Autor) Outra; Claudia Bessa (Autor) Outra; Lucilia Saraiva (Autor) FFUP

Revista

Título: FEMS Yeast Research

Vol. 12

Páginas: 755-760

ISSN: 1567-1356

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-45H

DOI: 10.1111/j.1567-1364.2012.00825.x

Abstract (EN): Yeast revealed to be a versatile organism for studying endocytosis. Here, inhibition of endocytosis by H2O2 and its correlation with apoptotic cell death were ascertained in Saccharomyces cerevisiae. We found that H2O2 causes alterations in vacuolar morphology and a concentration-dependent inhibition of endocytosis. We also found that H2O2-induced endocytosis inhibition is a reversible process that occurs in the early phase of the apoptotic cascade, preceding chromatin condensation and DNA fragmentation. Additionally, mutants affecting early steps of the endocytic pathway display sensitivity to H2O2. As endocytosis inhibition was also observed with acetic acid, it may be a broader cellular dysfunction of oxidative stress-induced toxicity in yeast.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6