Functional characterization of Arabidopsis thaliana transthyretin-like protein

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Joao Pessoa (Autor) Outra; Zsuzsa Sarkany (Autor) Outra; Frederico Ferreira da Silva (Autor) Outra; Sonia Martins (Autor) Outra; Maria R Almeida (Autor) ICBAS; Jianming M Li (Autor) Outra; Ana M Damas (Autor) ICBAS

Revista

Título: BMC Plant Biology

Vol. 10

Página Final: 30

ISSN: 1471-2229

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 25 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-93J

DOI: 10.1186/1471-2229-10-30

Abstract (EN): Background: Arabidopsis thaliana transthyretin-like (TTL) protein is a potential substrate in the brassinosteroid signalling cascade, having a role that moderates plant growth. Moreover, sequence homology revealed two sequence domains similar to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (N-terminal domain) and 5-hydroxyisourate (5-HIU) hydrolase (C-terminal domain). TTL is a member of the transthyretin-related protein family (TRP), which comprises a number of proteins with sequence homology to transthyretin (TTR) and the characteristic C-terminal sequence motif Tyr-Arg-Gly-Ser. TRPs are single domain proteins that form tetrameric structures with 5-HIU hydrolase activity. Experimental evidence is fundamental for knowing if TTL is a tetrameric protein, formed by the association of the 5-HIU hydrolase domains and, in this case, if the structural arrangement allows for OHCU decarboxylase activity. This work reports about the biochemical and functional characterization of TTL. Results: The TTL gene was cloned and the protein expressed and purified for biochemical and functional characterization. The results show that TTL is composed of four subunits, with a moderately elongated shape. We also found evidence for 5-HIU hydrolase and OHCU decarboxylase activities in vitro, in the full-length protein. Conclusions: The Arabidopsis thaliana transthyretin-like (TTL) protein is a tetrameric bifunctional enzyme, since it has 5-HIU hydrolase and OHCU decarboxylase activities, which were simultaneously observed in vitro.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10